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Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
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JoVE Journal 生化学
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

DOI:
10.3791/60659-v

•

09:15 min

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January 21, 2020

•

,
1Biomedical Engineering Program,Ohio University, 2Department of Chemical and Biomolecular Engineering,Ohio University

章

  • 00:04標題
  • 00:42Erythrocyte Isolation from Whole Blood
  • 02:19Treatment of Erythrocytes with Ionomycin and Measurement of Hemolysis
  • 04:43Annexin-V Binding Assay
  • 05:48Flow Cytometry
  • 06:47Results: Flow Cytometry and Confocal Fluorescence Microscopy
  • 08:12Conclusion

概要

自動翻訳

自動翻訳

A protocol for the induction of eryptosis, programmed cell death in erythrocytes, using the calcium ionophore, ionomycin, is provided. Successful eryptosis is evaluated by monitoring the localization phosphatidylserine in the membrane outer leaflet. Factors affecting the success of the protocol have been examined and optimal conditions provided.

タグ

EryptosisRed Blood CellsCalcium IonophoreIonomycinRinger SolutionGlucose-free BufferHematocritCell SuspensionCentrifugationIncubation

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