ジャーナル
/
発生生物学
/
Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

DOI:
10.3791/55606-v

•

09:53 min

•

June 03, 2017

•

, , , , ,
1生物医科学科,NYITCOM, 2Department of Basic Sciences,NYITCOM-A-State

章

  • 00:05標題
  • 01:05Maintaining Embryos and Mounting Zebrafish for Live-imaging
  • 02:59Laser Ablation
  • 06:53Results: Acute Kidney in Zebrafish by Precise Cellular Ablation
  • 08:42Conclusion

概要

自動翻訳

自動翻訳

This work presents a new in vivo model of segmental kidney injury using kidney GFP transgenic zebrafish. The model allows for the induction of the targeted ablation of kidney epithelial cells to show the cellular mechanisms of nephron injury and repair.

タグ

Kidney AblationZebrafishAcute Kidney InjuryNephron InjuryKidney RegenerationSpatial Temporal ControlLive ImagingPhoto AblationConfocal MicroscopyAgarose Embedding

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Mechanical Vessel Injury in Zebrafish Embryos

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Technique to Target Microinjection to the Developing Xenopus Kidney

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Surgical Ablation Assay for Studying Eye Regeneration in Planarians

Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Read Article