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Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

DOI:
10.3791/55606-v

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09:53 min

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June 03, 2017

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, , , , ,
1Department of Biomedical Sciences,NYITCOM, 2Department of Basic Sciences,NYITCOM-A-State

Kapitel

  • 00:05Titel
  • 01:05Maintaining Embryos and Mounting Zebrafish for Live-imaging
  • 02:59Laser Ablation
  • 06:53Results: Acute Kidney in Zebrafish by Precise Cellular Ablation
  • 08:42Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This work presents a new in vivo model of segmental kidney injury using kidney GFP transgenic zebrafish. The model allows for the induction of the targeted ablation of kidney epithelial cells to show the cellular mechanisms of nephron injury and repair.

Tags

Kidney AblationZebrafishAcute Kidney InjuryNephron InjuryKidney RegenerationSpatial Temporal ControlLive ImagingPhoto AblationConfocal MicroscopyAgarose Embedding

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