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Un metodo per misurare il metabolismo in Ordinati sottopopolazioni di cellule Comunità complessi utilizzando Stable Isotope Tracing
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生化学
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JoVE Journal 生化学
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
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Un metodo per misurare il metabolismo in Ordinati sottopopolazioni di cellule Comunità complessi utilizzando Stable Isotope Tracing

8,094 Views

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07:41 min

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February 04, 2017

DOI:

10.3791/55011-v

DOI:
10.3791/55011-v

•

07:41 min
February 04, 2017

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3 Views
, , , , ,
1Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, 2Center for Molecular Medicine,Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, 4医学部,University of California San Diego

概要

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This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

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Metabolite ExtractionCell SortingStable Isotope Tracing代謝Cell SubpopulationsCell CultureMass Spectrometry

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Cite this Article

Roci, I., Gallart-Ayala, H., Watrous, J., Jain, M., Wheelock, C. E., Nilsson, R. A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing. J. Vis. Exp. (120), e55011, doi:10.3791/55011 (2017).

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