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Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
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December 03, 2016
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We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.
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Houston, D. A., Staines, K. A., MacRae, V. E., Farquharson, C. Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. J. Vis. Exp. (118), e54978, doi:10.3791/54978 (2016).
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