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T细胞受体删除环同时定量(TRECs)和K-删除重组删除环(KRECs)通过实时PCR
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

T细胞受体删除环同时定量(TRECs)和K-删除重组删除环(KRECs)通过实时PCR

DOI:

14:14 min

December 06, 2014

, , , , , ,

  • 00:05標題
  • 02:34Preparation of Triple-insert Plasmid
  • 04:49Standard Curve Preparation and Real Time PCR for Quantification of TRECS and KRECS
  • 07:25Calculations
  • 11:59Results: TREC and KREC Quantification in Healthy Individuals
  • 12:54Conclusion

概要

自動翻訳

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

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