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Quantification simultanée de T-Cell Receptor Cercles excision (TREC) et cercles K-Suppression recombinaison Excision (KRECs) par PCR en temps réel
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JoVE Journal 免疫学と感染
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
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Quantification simultanée de T-Cell Receptor Cercles excision (TREC) et cercles K-Suppression recombinaison Excision (KRECs) par PCR en temps réel

16,194 Views

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14:14 min

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December 06, 2014

DOI:

10.3791/52184-v

DOI:
10.3791/52184-v

•

14:14 min
December 06, 2014

•

3 Views
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1CREA, Diagnostics Department,Spedali Civili di Brescia

概要

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Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

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Cite this Article

Sottini, A., Serana, F., Bertoli, D., Chiarini, M., Valotti, M., Vaglio Tessitore, M., Imberti, L. Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR. J. Vis. Exp. (94), e52184, doi:10.3791/52184 (2014).

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