Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Alessandra Sottini; Federico Serana; Diego Bertoli; Marco Chiarini; Monica Valotti; Marion Vaglio Tessitore; Luisa Imberti

doi:10.3791/52184

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Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

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JoVE Journal 免疫学と感染

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

16,194 Views

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14:14 min

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December 06, 2014

DOI:

10.3791/52184-v

DOI:

10.3791/52184-v

•

14:14 min

December 06, 2014

•

3 Views

Alessandra Sottini¹, Federico Serana¹, Diego Bertoli¹, Marco Chiarini¹, Monica Valotti¹, Marion Vaglio Tessitore¹, Luisa Imberti¹

¹CREA, Diagnostics Department,Spedali Civili di Brescia

概要

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

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Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

概要

Automatically generated

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