ジャーナル
/
免疫学と感染
/
כימות סימולטני של מעגלי קולטן כריתת T-Cell (TRECs) ומעגלי K-מחיקת רקומבינציה כריתה (KRECs) על ידי בזמן אמת PCR
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
Please note that all translations are automatically generated. Click here for the English version.

כימות סימולטני של מעגלי קולטן כריתת T-Cell (TRECs) ומעגלי K-מחיקת רקומבינציה כריתה (KRECs) על ידי בזמן אמת PCR

16,194 Views

•

14:14 min

•

December 06, 2014

DOI:

10.3791/52184-v

DOI:
10.3791/52184-v

•

14:14 min
December 06, 2014

•

3 Views
, , , , , ,
1CREA, Diagnostics Department,Spedali Civili di Brescia

概要

Automatically generated

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

Explore More Videos

T-cell Receptor Excision Circles (TRECs)K-deleting Recombination Excision Circles (KRECs)Real-time PCRSimultaneous QuantificationImmune ReconstitutionBone Marrow TransplantationImmune DeficienciesImmunomodulating Drugs

Read Article

Cite this Article

Sottini, A., Serana, F., Bertoli, D., Chiarini, M., Valotti, M., Vaglio Tessitore, M., Imberti, L. Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR. J. Vis. Exp. (94), e52184, doi:10.3791/52184 (2014).

Download .ris file

Copy

Share Video

.

Copy