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Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
1Centre for Integrative Physiology, School of Biomedical Sciences,The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine,Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems,The University of Edinburgh
章
- 00:05標題
- 01:17Fabrication of Parylene Patterns on SiO2
- 03:31Chip Cleaning and Activation
- 04:26Plating Cell Line On-chip
- 05:12Results: Cell Patterning on Photolithographically-defined Parylene-C
- 06:49Conclusion
This protocol describes a microfabrication-compatible method for cell patterning on SiO2. A predefined parylene-C design is photolithographically printed on SiO2 wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO2 regions.
タグ
Cell PatterningParylene-CSiO2 SubstratesPhotolithographyMulti-electrode ArraysLab On A ChipCell AdhesionCell-specific Adhesion MoleculesPrimary Murine Hippocampal CellsHEK 293 Cell LineHuman Neuron-like Teratocarcinoma Cell LinePrimary Murine Cerebellar Granule CellsPrimary Human Glioma-derived Stem-like Cells
