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Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
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07:19 min
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March 07, 2014
DOI:
1Centre for Integrative Physiology, School of Biomedical Sciences,The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine,Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems,The University of Edinburgh
Summary
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This protocol describes a microfabrication-compatible method for cell patterning on SiO2. A predefined parylene-C design is photolithographically printed on SiO2 wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO2 regions.
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Cite this Article
Hughes, M. A., Brennan, P. M., Bunting, A. S., Shipston, M. J., Murray, A. F. Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates. J. Vis. Exp. (85), e50929, doi:10.3791/50929 (2014).
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