このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Vergleichende In vivo Studium der gp96 Adjuvantizität in the Frog Xenopus laevis</em
章
- 00:00標題
- 01:24Introduction
- 02:12Elicitation and Harvest of Peritoneal Leukocytes (PLs)
- 03:43Pulsing and Adoptive Transfer of LG-6 PLs into Naive LG-6 Recipients
- 05:20Skin Graft Assay
- 07:14Tumor Transplantation Assay
- 07:56Collecting Blood Leukocytes
- 08:58Skin Graft and Tumor Transplantation Results
- 10:34Conclusion
Der Frosch<em> Xenopus laevis</em> Bietet eine attraktive Alternative Nicht-Säuger-Modell für die Erkundung der Fähigkeit des Hitzeschock-Proteins, wie gp96, Antigen-spezifische CD8 T-Zell-Reaktionen zu fördern. Wir präsentieren Methoden zur Untersuchung<em> In vivo</em> Erleichterung der Cross-Präsentation von Haut-und Tumor-Antigene von gp96.
タグ
Gp96 AdjuvanticityComparative In Vivo StudyFrog Xenopus LaevisHeat Shock ProteinsChaperoned AntigensInnate And Adaptive T Cell ResponsesXenopus Skin Graft RejectionMHC Class Ia Restricted T Cell ResponsesTumors Down-regulating Class Ia MoleculesAdoptive Cell Transfer AssayPeritoneal LeukocytesAntigen Presenting Cells (APCs)CD8 T Cell ResponsesMinor Histocompatibility Skin AntigensXenopus Thymic Tumor 15/0MethodologyElicitationPulsingAdoptive TransferSkin Graft Transplantation AssayTumor Transplantation AssayHarvesting Peripheral Blood LeukocytesFlow CytometryProliferation Assays
