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小鼠 体内 胎盘靶向CRISPR操作
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Genetica
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JoVE Journal Genetica
Mouse In Vivo Placental Targeted CRISPR Manipulation
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小鼠 体内 胎盘靶向CRISPR操作

1,975 Views

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07:39 min

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April 14, 2023

DOI:

10.3791/64760-v

DOI:
10.3791/64760-v

•

07:39 min
April 14, 2023

•

10 Views
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1Interdisciplinary Graduate Program in Genetics,University of Iowa, 2Department of Psychiatry, Carver College of Medicine,University of Iowa, 3Iowa Neuroscience Institute, Carver College of Medicine,University of Iowa, 4Hawk-IDDRC,University of Iowa

Summary

Automatically generated

在这里，我们描述了一种时间特异性方法，以有效操纵 体内小鼠胎盘中的关键发育途径。这是通过在胚胎第12.5天将CRISPR质粒注射和电穿孔到妊娠母亲的胎盘中进行的。

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Cite this Article

Carver, A. J., Taylor, R. J., Stevens, H. E. Mouse In Vivo Placental Targeted CRISPR Manipulation. J. Vis. Exp. (194), e64760, doi:10.3791/64760 (2023).

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