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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
 

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Article DOI: 10.3791/62510-v • 08:57 min • July 17th, 2021
July 17th, 2021 •
1, 1
1Structural Genomics Consortium, University of Toronto

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The baculovirus expression vector system (BEVS) is a robust platform for expression screening and production of protein arginine methyltransferases (PRMTs) to be used for biochemical, biophysical, and structural studies. Milligram quantities of material can be produced for the majority of PRMTs and other proteins of interest requiring a eukaryotic expression platform.

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Recombinant PRMT Proteins Baculovirus Expression Vector System Expression Screening Protein Arginine Methyltransferases Large-scale Production Milligram Quantity Biochemical Studies Biophysical Studies Structural Studies Medium Supported Mode Cost Efficient Serum-free Insect Media SF9 Cells Sterile Reagent Reservoir Programmable Multi-channel Pipette 24-well Plate Transfection Mix Transfection Efficiency Cell Only Control Infection Signs Assessment 27 Degrees Incubation Unsupplemented Insect Medium Diluted Transaction Reag
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