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Biochemistry

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JoVE Journal Biochemistry
Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
 

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Article DOI: 10.3791/64322-v • 06:26 min • January 6th, 2023
January 6th, 2023 •
1, 1, 1, 1
1Peak Proteins Ltd.

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The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.

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Fluorescent Size Exclusion Chromatography SEC Profile Membrane Protein Quality FSEC Sample Preparation Solubilization Buffer Detergent Stock Low-speed Centrifugation High-speed Centrifugation
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