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Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
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Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
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April 27, 2019
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SUMO is an essential and highly conserved, small ubiquitin-like modifier protein. In this protocol we are describing the use of a stress-tolerant recombinant SUMO-trapping protein (kmUTAG) to visualize native, untagged SUMO conjugates and their localization in a variety of cell types.
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Yin, R., Harvey, C., Shakes, D. C., Kerscher, O. Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins. J. Vis. Exp. (146), e59576, doi:10.3791/59576 (2019).
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