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Journal / Developmental Biology / 建立高通量表皮球体培养系统，以模拟角膜细胞干细胞可塑性…
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Developmental Biology

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JoVE Journal Developmental Biology
建立高通量表皮球体培养系统，以模拟角膜细胞干细胞可塑性
 
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建立高通量表皮球体培养系统，以模拟角膜细胞干细胞可塑性

Article DOI: 10.3791/62182-v • 10:03 min • January 30th, 2021
January 30th, 2021 •
1,2, 3, 4, 5, 6, 1
1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina

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在这里，我们描述了在3D悬架培养中系统地培养表皮球体的协议。本协议具有广泛的应用，用于各种上皮组织类型和几个人类疾病和条件的建模。

Tags

发育生物学， 第 167 期， Anoikis， 细胞可塑性， 上皮， 表皮干细胞， 表皮球， 人类角膜细胞， 球体， 三维培养， 伤口愈合
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