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Journal / Developmental Biology / Etablering af en høj gennemløb Epidermal Spheroid …
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Developmental Biology

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JoVE Journal Developmental Biology
Etablering af en høj gennemløb Epidermal Spheroid Kultur System til Model Keratinocyte Stamcelle Plasticitet
 
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Etablering af en høj gennemløb Epidermal Spheroid Kultur System til Model Keratinocyte Stamcelle Plasticitet

Article DOI: 10.3791/62182-v • 10:03 min • January 30th, 2021
January 30th, 2021 •
1,2, 3, 4, 5, 6, 1
1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina

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Her beskriver vi en protokol til systematisk dyrkning af epidermale sfæroider i 3D-suspensionskultur. Denne protokol har vidtrækkende anvendelser til brug i en række epitelvævstyper og til modellering af flere menneskelige sygdomme og tilstande.

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Udviklingsbiologi Problem 167 Anoikis celleplastik epitel epidermale stamceller epidermosfære humane keratinocytter sfæroider 3D-kultur sårheling
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