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成人耳皮肤全安装共焦显微术: 一种研究神经血管分支形态发生和免疫细胞分布的模型系统
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
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成人耳皮肤全安装共焦显微术: 一种研究神经血管分支形态发生和免疫细胞分布的模型系统

DOI:
10.3791/57406-v

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08:42 min

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March 29, 2018

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, ,
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Capitoli

  • 00:04Titolo
  • 01:03Adult Mouse Skin Collection
  • 02:50Whole-mount Immunohistochemical Staining of Mouse Ear Skin
  • 05:16Mounting Ear Skin on Slide
  • 06:42Confocal Microscopy
  • 07:13Results: Neuro-vascular Branching Morphogenesis and Immune Cell Distribution in Adult Ear Skin
  • 07:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们描述了一个高分辨率全安装成像方法在整个成年小鼠耳皮肤, 这使我们能够想象分支形态发生和模式的周围神经和血管, 以及免疫细胞分布。

Tags

Whole-mountConfocal MicroscopyEar SkinNeuro-vascular BranchingMorphogenesisImmune Cell DistributionPeripheral NervesBlood VesselsImmunohistochemistrySkin Preparation3D VisualizationNormal And Pathological Conditions

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