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从小鼠垂体中开发类器官作为 体外 模型以探索垂体干细胞生物学
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
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从小鼠垂体中开发类器官作为 体外 模型以探索垂体干细胞生物学

DOI:
10.3791/63431-v

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09:48 min

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February 25, 2022

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, ,
1Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute,KU Leuven (University of Leuven)

Capitoli

  • 00:04Introduction
  • 00:53Isolation of Mouse Anterior Lobe
  • 01:57Dissociation of Pituitary into Single Cells
  • 04:28Establishment and Culturing of Anterior Lobe Derived Organoids
  • 07:31Results: Establishment and Validation of Anterior Lobe Derived Organoids
  • 09:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

垂体是人体内分泌系统的关键调节因子。本文描述了从小鼠垂体中开发类器官作为一种新的3D 体外 模型，以研究腺体的干细胞群，其中的生物学和功能仍然知之甚少。

Tags

OrganoidsMouse PituitaryIn Vitro ModelPituitary Stem Cell BiologyPituitary RemodelingNeonatal MaturationAgingTumor GrowthCell IsolationTrypsinDNAseCell CultureMedium

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