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从人类诱导多能干细胞诱导的体外神经肌肉结点
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
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从人类诱导多能干细胞诱导的体外神经肌肉结点

DOI:
10.3791/61396-v

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06:01 min

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December 03, 2020

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, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Capitoli

  • 00:04Introduction
  • 00:59Induced Pluripotent Stem Cell (iPSC) Culture
  • 02:00iPSC Differentiation
  • 03:04Neuromuscular Junction (NMJ) Muscle Contraction and Curare Treatment
  • 03:47Results: Representative NMJ Phenotypic, Morphological, and Functional Characterization
  • 05:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提供一个协议，从人类诱导多能干细胞（iPSCs）生成体外NMJ。该方法在1个月内可以诱导形态成熟、功能良好的NMJs。由此产生的NMJs可能用于模拟相关疾病、研究病理机制或筛选药物化合物以进行治疗。

Tags

Human Induced Pluripotent Stem CellsNeuromuscular JunctionMotor NeuronsSkeletal MuscleSchwann CellsSpinal Muscular AtrophyCell DifferentiationCell CultureCell DetachmentExtracellular MatrixDoxycyclineDifferentiation MediumMyogenic Differentiation MediumNeuromuscular Junction Medium

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