Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.
Lin, C., Yoshida, M., Li, L., Saito, M. K. In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (166), e61396, doi:10.3791/61396 (2020).