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Biologia do Desenvolvimento
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In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

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06:01 min

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December 03, 2020

DOI:

10.3791/61396-v

DOI:
10.3791/61396-v

•

06:01 min
December 03, 2020

•

5 Views
, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Summary

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Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

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Lin, C., Yoshida, M., Li, L., Saito, M. K. In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (166), e61396, doi:10.3791/61396 (2020).

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