Rivista
/
Biologia dello sviluppo
/
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

5,335 Views

•

06:01 min

•

December 03, 2020

DOI:

10.3791/61396-v

DOI:
10.3791/61396-v

•

06:01 min
December 03, 2020

•

5 Views
, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Summary

Automatically generated

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

Explore More Videos

Human Induced Pluripotent Stem CellsNeuromuscular JunctionMotor NeuronsSkeletal MuscleSchwann CellsSpinal Muscular AtrophyCell DifferentiationCell CultureCell DetachmentExtracellular MatrixDoxycyclineDifferentiation MediumMyogenic Differentiation MediumNeuromuscular Junction Medium

Read Article

Cite this Article

Lin, C., Yoshida, M., Li, L., Saito, M. K. In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (166), e61396, doi:10.3791/61396 (2020).

Download .ris file

Copy

Share Video

.

Copy