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Immunology and Infection

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JoVE Journal Immunology and Infection
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
 

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Article DOI: 10.3791/59609-v • 10:06 min • July 10th, 2019
July 10th, 2019 •
1, 2, 2, 2
1State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 2Institute for Immunology, School of Medicine, Tsinghua University

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We present a protocol to functionalize atomic force microscope (AFM) cantilevers with a single T cell and bead particle for immunological studies. Procedures to probe single-pair T cell-dendritic cell binding by AFM and to monitor the real-time cellular response of macrophages to a single solid particle by AFM with fluorescence imaging are shown.

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Functionalization Atomic Force Microscope Cantilevers Single T Cells Single-Particle Immunological Single-Cell Force Spectroscopy Functionalize AFM Cantilevers Single-pair Dendritic Cell-T Cell Interactions Cellular Responses Biocompatible Glue Eukaryotic Cells Baseline Activation Stage Immune Cell Activation Immune Synapse Formation Cell-cell Interactions Cell-surface Interactions IL-2 Pretreatment Glass Coverslips DC2.4 Cells Humidified Chamber
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