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Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
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JoVE Journal Immunologia e infezioni
Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

11,673 Views

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08:47 min

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April 05, 2019

DOI:

10.3791/59400-v

DOI:
10.3791/59400-v

•

08:47 min
April 05, 2019

•

26 Views
, ,
1Department of Biochemistry and Molecular Biology, Center for Molecular Medicine and Complex Carbohydrate Research Center,University of Georgia

Summary

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This opsonophagocytic killing assay is used to compare the ability of phagocytic immune cells to respond to and kill bacteria based on different treatments and/or conditions. Classically, this assay serves as the gold standard for assessing effector functions of antibodies raised against a bacterium as opsonin.

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Cite this Article

Paschall, A. V., Middleton, D. R., Avci, F. Y. Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens. J. Vis. Exp. (146), e59400, doi:10.3791/59400 (2019).

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