Here we describe the protocols for applying defined mechanical loads to mouse calves and for monitoring the concomitant intramuscular pressure changes. The experimental systems that we have developed can be useful for investigating the mechanism behind the beneficial effects of physical exercise and massage.
Sakitani, N., Maekawa, T., Saitou, K., Suzuki, K., Murase, S., Tokunaga, M., Yoshino, D., Sawada, K., Takashima, A., Nagao, M., Ogata, T., Sawada, Y. Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes. J. Vis. Exp. (151), e59475, doi:10.3791/59475 (2019).