Rivista
/
Neuroscienze
/
Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

DOI:
10.3791/59232-v

•

08:52 min

•

May 23, 2019

•

, , ,
1Departments of Bioengineering,University of Washington, 2Washington National Primate Research Center, 3Department of Electrical and Computer Engineering,University of Washington, 4Department of Physiology and Center for Integrative Neuroscience,University of California, San Francisco

Capitoli

  • 00:04Titolo
  • 00:39MR-compatible Chamber Implantation
  • 02:05Viral Vector Delivery
  • 06:18Results: Validation of Viral Expression
  • 07:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

Tags

Convection Enhanced DeliveryOptogeneticAdeno-associated Viral VectorCortexRhesus MacaqueMRICraniotomyDuraMR Compatible ChamberInjection GridSalineSterile Absorbable Gel FoamVitamin E Capsule

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Intracranial Injection of Adeno-associated Viral Vectors

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Optogenetic Functional MRI

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Automated Segmentation of Cortical Grey Matter from T1-Weighted MRI Images

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Delivery of Antibodies into the Murine Brain via Convection-enhanced Delivery

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

Read Article