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Fluorimetric Techniques for the Assessment of Sperm Membranes
Capitoli
- 00:04Titolo
- 00:55Sperm Sample Preparation
- 02:03Technique #1: Simultaneous Assessment of Sperm Membranes Using Multiple Flourescent Probes
- 04:34Technique #2: Assessment of Sperm Membranes with Ready-to-use Kits and Flow Cytometry
- 06:58Results: Evaluation of Semen Quality
- 08:16Conclusion
Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.
