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蛍光光度法による精子膜評価技術

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Fluorimetric Techniques for the Assessment of Sperm Membranes

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蛍光光度法による精子膜評価技術

12,853 Views

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08:58 min

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November 28, 2018

DOI:

10.3791/58622-v

DOI:

10.3791/58622-v

•

08:58 min

November 28, 2018

•

27 Views

Alisa Komsky-Elbaz^1,2, Zvi Roth^1,2

¹Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, ²Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem

Summary

精子膜の完全性、精子の受精能力に関連付けられている携帯電話の機能を評価する方法論を紹介します。精子の膜の蛍光光度法による評価のための 3 つの技術について述べる: 高度な精子専用フローサイトメトリー、蛍光顕微鏡、特定の蛍光プローブの同時染色します。方法論を組み合わせることの紹介も。

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