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Única molécula fluorescência In Situ Hybridization (smFISH) análise em Budding fermento crescimento vegetativo e meiose
JoVE Journal
Genetica
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JoVE Journal Genetica
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Única molécula fluorescência In Situ Hybridization (smFISH) análise em Budding fermento crescimento vegetativo e meiose

18,731 Views

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09:28 min

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May 25, 2018

DOI:

10.3791/57774-v

DOI:
10.3791/57774-v

•

09:28 min
May 25, 2018

•

7 Views
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1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Summary

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Esta molécula fluorescência em situ da hibridação protocolo é otimizado para quantificar o número de moléculas de RNA em leveduras brotamento durante a meiose e crescimento vegetativo.

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Cite this Article

Chen, J., McSwiggen, D., Ünal, E. Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. J. Vis. Exp. (135), e57774, doi:10.3791/57774 (2018).

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