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単一分子蛍光In-situハイブリダイゼーション (smFISH) における出芽酵母の栄養増殖と減数分裂
JoVE Journal
Genetica
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JoVE Journal Genetica
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
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単一分子蛍光In-situハイブリダイゼーション (smFISH) における出芽酵母の栄養増殖と減数分裂

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09:28 min

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May 25, 2018

DOI:

10.3791/57774-v

DOI:
10.3791/57774-v

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09:28 min
May 25, 2018

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7 Views
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1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Summary

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この単一分子蛍光その場で交配のプロトコルは、栄養生長と減数分裂中出芽酵母の RNA 分子の数を数値化に最適です。

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Cite this Article

Chen, J., McSwiggen, D., Ünal, E. Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. J. Vis. Exp. (135), e57774, doi:10.3791/57774 (2018).

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