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Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

13,131 Views

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09:41 min

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October 17, 2017

DOI:

10.3791/56293-v

DOI:
10.3791/56293-v

•

09:41 min
October 17, 2017

•

19 Views
, , , ,
1Centre for Biomedical Research,Burnet Institute, 2School of Health and Biomedical Sciences,RMIT University, 3Department of Infectious Diseases,Monash University, 4University of California, Los Angeles

Summary

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We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

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Cite this Article

Angelovich, T. A., Hearps, A. C., Maisa, A., Kelesidis, T., Jaworowski, A. Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions. J. Vis. Exp. (128), e56293, doi:10.3791/56293 (2017).

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