Journal
/
Inmunología y contagio
/
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

DOI:
10.3791/56293-v

•

09:41 min

•

October 17, 2017

•

, , , ,
1Centre for Biomedical Research,Burnet Institute, 2School of Health and Biomedical Sciences,RMIT University, 3Department of Infectious Diseases,Monash University, 4University of California, Los Angeles

Capítulos

  • 00:05Título
  • 00:50Culturing HUVEC Monolayer on Collagen Gels
  • 03:38Activation of HUVEC Monolayer and Isolation/Activation of Monocytes for Transmigration
  • 05:44Quantification of Foam Cells and Macrophages by Microscopy or Flow Cytometry
  • 07:42Results: Characterizing the Transmigrating Mice
  • 09:09Conclusion

Summary

Traducción Automática

Traducción Automática

We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

Tags

Monocyte TransmigrationFoam Cell FormationChronic Inflammatory ConditionsCardiovascular DiseaseAtherosclerosisEndothelial CellsCell CultureCollagen GelHUVECMicroscopyFlow Cytometry

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli

Read Article