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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
 

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Article DOI: 10.3791/56293-v • 09:41 min • October 17th, 2017
October 17th, 2017 •
1,2, 1,3, 1, 4, 1,3
1Centre for Biomedical Research, Burnet Institute, 2School of Health and Biomedical Sciences, RMIT University, 3Department of Infectious Diseases, Monash University, 4University of California, Los Angeles

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We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

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Quantification Monocyte Transmigration Foam Cell Formation Chronic Inflammatory Conditions Cardiovascular Field Atherogenic Potential Acute Coronary Artery Disease Clinical Samples Fresh Whole Blood Stored Patient Cohorts Microscopy Flow Cytometry Polymerized Collagen Gels Sodium Hydroxide M199 Acidic Acid Fibrous Collagen 96-well Tissue Culture Plate Dulbecco PBS Incubation Supplemented M199 Human Umbilical Vein Endothelial Cells
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