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Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
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Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

13,129 Views

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09:41 min

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October 17, 2017

DOI:

10.3791/56293-v

DOI:
10.3791/56293-v

•

09:41 min
October 17, 2017

•

19 Views
, , , ,
1Centre for Biomedical Research,Burnet Institute, 2School of Health and Biomedical Sciences,RMIT University, 3Department of Infectious Diseases,Monash University, 4University of California, Los Angeles

Summary

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We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

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Angelovich, T. A., Hearps, A. C., Maisa, A., Kelesidis, T., Jaworowski, A. Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions. J. Vis. Exp. (128), e56293, doi:10.3791/56293 (2017).

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