Rivista
/
Biologia dello sviluppo
/
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

DOI:
10.3791/55918-v

•

10:45 min

•

July 26, 2017

•

, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Capitoli

  • 00:05Titolo
  • 01:07Dissociation Protocol
  • 02:42Antibody Staining and Cell Sorting
  • 04:39Transfection
  • 05:57Ca2+ Imaging in Differentiated Cells and Image Analysis
  • 08:50Results: Myoblast Characterization and a SOCE Experiment
  • 09:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.

Tags

Human MyoblastsMyogenic DifferentiationStore-operated Calcium EntrySkeletal MuscleMuscle RegenerationIonic ChannelsTranscription FactorsMuscular DystrophiesTrypsinCell CultureCell IsolationCell Staining

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Read Article