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Isolierung von menschlichen Myoblasten, Beurteilung der myogenen Differenzierung und speicherbetriebenen Kalziumeintrittsmessung
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Isolierung von menschlichen Myoblasten, Beurteilung der myogenen Differenzierung und speicherbetriebenen Kalziumeintrittsmessung

9,834 Views

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10:45 min

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July 26, 2017

DOI:

10.3791/55918-v

DOI:
10.3791/55918-v

•

10:45 min
July 26, 2017

•

3 Views
, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Summary

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Hier beschreiben wir eine Methodik, um eine reine Population von menschlichen Myoblasten aus erwachsenem Muskelgewebe zu erhalten. Diese Zellen werden verwendet, um die In-vitro- Skelettmuskeldifferenzierung zu untersuchen und insbesondere Proteine ​​zu untersuchen, die an der Ca 2+ -Signalisierung beteiligt sind.

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Cite this Article

Laumonier, T., Koenig, S., Saüc, S., Frieden, M. Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement. J. Vis. Exp. (125), e55918, doi:10.3791/55918 (2017).

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