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精确的细胞消融方法建模发展中的斑马鱼急性肾损伤
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
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精确的细胞消融方法建模发展中的斑马鱼急性肾损伤

DOI:
10.3791/55606-v

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09:53 min

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June 03, 2017

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, , , , ,
1Department of Biomedical Sciences,NYITCOM, 2Department of Basic Sciences,NYITCOM-A-State

Capitoli

  • 00:05Titolo
  • 01:05Maintaining Embryos and Mounting Zebrafish for Live-imaging
  • 02:59Laser Ablation
  • 06:53Results: Acute Kidney in Zebrafish by Precise Cellular Ablation
  • 08:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

这项工作提出了使用肾GFP转基因斑马鱼的节段性肾损伤的新的体内模型。该模型允许诱导肾上皮细胞的靶向消融以显示肾单位损伤和修复的细胞机制。

Tags

Kidney AblationZebrafishAcute Kidney InjuryNephron InjuryKidney RegenerationSpatial Temporal ControlLive ImagingPhoto AblationConfocal MicroscopyAgarose Embedding

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