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Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
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Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
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April 13, 2017
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Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.
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Sarkar, S., Malovic, E., Plante, B., Zenitsky, G., Jin, H., Anantharam, V., Kanthasamy, A., Kanthasamy, A. G. Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility. J. Vis. Exp. (122), e55364, doi:10.3791/55364 (2017).
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