Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Rapid and Refined CD11b Magnetic Isolation of…
JoVE Journal
Neuroscience

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Neuroscience
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
 

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Article DOI: 10.3791/55364-v • 07:54 min • April 13th, 2017
April 13th, 2017 •
*1, *1, 1, 1, 1, 1, 1, 1
1Biomedical Sciences & Iowa Center for Advanced Neurotoxicology, Iowa State University
* These authors contributed equally

Chapitres

résumé
Automatic Translation

Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.

Tags

CD11b Magnetic Isolation Primary Microglia Purity Versatility In Vitro Experimentation Neuroscience Neural Inflammation Technique Isolation Time Viability Decapitated Heads Micro-dissecting Scissors Meninges Brain Transfer Trypsin EDTA Incubation Growth Medium Homogenize Pipette
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter