/
/
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
9,484 Views
•
07:54 min
•
April 13, 2017
DOI:
Summary
Automatically generated
Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.
Read Article
Cite this Article
Sarkar, S., Malovic, E., Plante, B., Zenitsky, G., Jin, H., Anantharam, V., Kanthasamy, A., Kanthasamy, A. G. Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility. J. Vis. Exp. (122), e55364, doi:10.3791/55364 (2017).
Copy