Journal
/
神经科学
/
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

DOI:
10.3791/55364-v

•

07:54 min

•

April 13, 2017

•

, , , , , , ,
1Biomedical Sciences & Iowa Center for Advanced Neurotoxicology,Iowa State University

Chapters

  • 00:05Title
  • 00:37Culture of Mixed Glial Cells
  • 02:14Isolation of Microglial Cells
  • 05:31Results: Characteristics of Primary Microglial Cultures
  • 07:24Conclusion

Summary

自动翻译

自动翻译

Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.

Tags

CD11bMagnetic IsolationPrimary MicrogliaPurityVersatilityPostnatal神经科学Neural InflammationRapid IsolationTrypsin EDTACell CultureCell StrainerTriturationCentrifugation

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism

Effects of Transcranial Alternating Current Stimulation on the Primary Motor Cortex by Online Combined Approach with Transcranial Magnetic Stimulation

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

Rapid Isolation of Dorsal Root Ganglion Macrophages

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Read Article