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Expressão Gênica transiente em tabaco usando Assembleia Gibson eo Gene Gun
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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Expressão Gênica transiente em tabaco usando Assembleia Gibson eo Gene Gun

20,145 Views

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12:02 min

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April 18, 2014

DOI:

10.3791/51234-v

DOI:
10.3791/51234-v

•

12:02 min
April 18, 2014

•

17 Views
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1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Systems Biology,Harvard Medical School, 3Department of Biotechnology,Delft University of Technology

Summary

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Este trabalho descreve um novo método para alvejar seletivamente organelas subcelulares nas plantas, analisadas usando o BioRad Gene Gun.

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Alternative SplicingChloroplastCytosolGibson AssemblyGFPGene GunMetabolic EngineeringMulti-organelle TargetingPeroxisomeSynthetic BiologyTobaccoTransient Gene Expression

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Cite this Article

Mattozzi, M. D., Voges, M. J., Silver, P. A., Way, J. C. Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun. J. Vis. Exp. (86), e51234, doi:10.3791/51234 (2014).

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