Rivista
/
Biologia
/
Electroporation에 대한 Murine 골수에서 조혈 세포 차 문화의 준비
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Please note that all translations are automatically generated. Click here for the English version.

Electroporation에 대한 Murine 골수에서 조혈 세포 차 문화의 준비

25,445 Views

•

08:15 min

•

January 06, 2009

DOI:

10.3791/1026-v

DOI:
10.3791/1026-v

•

08:15 min
January 06, 2009

•

7 Views
, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc

Summary

Automatically generated

이 절차는 murine 골수에서 조혈 세포 기본 문화를 확립하고 진 Pulser MXCell의 electroporation 시스템을 사용 transfection하여 다음 방법을 설명합니다.

Explore More Videos

Primary Hematopoietic Cell CulturesMurine Bone MarrowElectroporationPlasmid DNASiRNAGene Pulser MXcell Electroporation SystemGene Pulser Electroporation BufferTransfectionNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsVideo DemonstrationFemurTibia

Read Article

Cite this Article

Kroeger, K., Collins, M., Ugozzoli, L. The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation. J. Vis. Exp. (23), e1026, doi:10.3791/1026 (2009).

Download .ris file

Copy

Share Video

.

Copy