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De vervaardiging van primaire hematopoietische cellen culturen uit Murine Bone Marrow voor Elektroporatie
JoVE Journal
Biologia
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JoVE Journal Biologia
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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De vervaardiging van primaire hematopoietische cellen culturen uit Murine Bone Marrow voor Elektroporatie

25,445 Views

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08:15 min

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January 06, 2009

DOI:

10.3791/1026-v

DOI:
10.3791/1026-v

•

08:15 min
January 06, 2009

•

7 Views
, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc

Summary

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Deze procedure wordt beschreven hoe de primaire hematopoietische celculturen te zetten vanaf knaagdier beenmerg en wordt gevolgd door transfectie met de Gene Puiser MXCell elektroporatie systeem.

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Kroeger, K., Collins, M., Ugozzoli, L. The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation. J. Vis. Exp. (23), e1026, doi:10.3791/1026 (2009).

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