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主电穿孔的小鼠骨髓造血细胞培养制备
JoVE Journal
Biologia
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JoVE Journal Biologia
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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主电穿孔的小鼠骨髓造血细胞培养制备

25,445 Views

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08:15 min

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January 06, 2009

DOI:

10.3791/1026-v

DOI:
10.3791/1026-v

•

08:15 min
January 06, 2009

•

7 Views
, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc

Summary

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此过程介绍了如何建立从小鼠骨髓造血的主要细胞培养和使用转基因脉冲MXCell电系统。

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Kroeger, K., Collins, M., Ugozzoli, L. The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation. J. Vis. Exp. (23), e1026, doi:10.3791/1026 (2009).

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