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主电穿孔的小鼠骨髓造血细胞培养制备

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

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主电穿孔的小鼠骨髓造血细胞培养制备

DOI:

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08:15 min

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January 06, 2009

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Kelly Kroeger, Michelle Collins, Luis Ugozzoli

¹Gene Expression Division,Bio-Rad Laboratories, Inc

Capitoli

00:00Titolo
00:08Introduction
00:43Harvesting Bone Marrow From Femurs and Tibiae
02:07Establishing Cultures
04:33Electroporating Cells
07:13Transfection Results
07:53Conclusion

Summary

Traduzione automatica

此过程介绍了如何建立从小鼠骨髓造血的主要细胞培养和使用转基因脉冲MXCell电系统。

Tags

Primary Hematopoietic Cell Cultures Murine Bone Marrow Electroporation Plasmid DNA SiRNA Gene Pulser MXcell Electroporation System Gene Pulser Electroporation Buffer Transfection Nucleic Acids Mammalian Cells Difficult-to-transfect Cells Stem Cells Video Demonstration Femur Tibia

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