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Mouse In Vivo Placental 표적 CRISPR 조작

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Génétique

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Journal JoVE Génétique

Mouse In Vivo Placental Targeted CRISPR Manipulation

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Mouse In Vivo Placental 표적 CRISPR 조작

1,975 Views

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07:39 min

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April 14, 2023

DOI:

10.3791/64760-v

DOI:

10.3791/64760-v

•

07:39 min

April 14, 2023

•

10 Views

Annemarie J. Carver^1,2,3, Robert J. Taylor^2,3, Hanna E. Stevens^1,2,3,4

¹Interdisciplinary Graduate Program in Genetics,University of Iowa, ²Department of Psychiatry, Carver College of Medicine,University of Iowa, ³Iowa Neuroscience Institute, Carver College of Medicine,University of Iowa, ⁴Hawk-IDDRC,University of Iowa

Summary

여기에서는 생체 내에서 마우스 태반의 중요한 발달 경로를 효과적으로 조작하는 시간별 방법을 설명합니다. 이것은 배아 12.5일째에 임신한 댐의 태반에 CRISPR 플라스미드를 주입하고 전기천공하여 수행됩니다.

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