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小鼠体内胎盘靶向CRISPR操作

Journal JoVE
Génétique

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Journal JoVE Génétique

Mouse In Vivo Placental Targeted CRISPR Manipulation

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小鼠体内胎盘靶向CRISPR操作

1,975 Views

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07:39 min

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April 14, 2023

DOI:

10.3791/64760-v

DOI:

10.3791/64760-v

•

07:39 min

April 14, 2023

•

10 Views

Annemarie J. Carver^1,2,3, Robert J. Taylor^2,3, Hanna E. Stevens^1,2,3,4

¹Interdisciplinary Graduate Program in Genetics,University of Iowa, ²Department of Psychiatry, Carver College of Medicine,University of Iowa, ³Iowa Neuroscience Institute, Carver College of Medicine,University of Iowa, ⁴Hawk-IDDRC,University of Iowa

Summary

在这里，我们描述了一种时间特异性方法，以有效操纵体内小鼠胎盘中的关键发育途径。这是通过在胚胎第12.5天将CRISPR质粒注射和电穿孔到妊娠母亲的胎盘中进行的。

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