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线粒体和内质视网膜成像由相关光和体积电子显微镜
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Biochimie
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Journal JoVE Biochimie
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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线粒体和内质视网膜成像由相关光和体积电子显微镜

12,413 Views

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09:21 min

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July 20, 2019

DOI:

10.3791/59750-v

DOI:
10.3791/59750-v

•

09:21 min
July 20, 2019

•

11 Views
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1Neural Circuits Research Group,Korea Brain Research Institute

Summary

Automatically generated

我们提出了一个方案,研究线粒体和内质神经在全细胞的分布后,使用相关的光和体积电子显微镜,包括抗坏血蜡过氧化物酶2和马萝卜过氧化物酶染色后,同一部分中具有或没有靶基因的细胞的序列切片,并通过电子显微镜进行序列成像。

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Cite this Article

Jung, M., Mun, J. Y. Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy. J. Vis. Exp. (149), e59750, doi:10.3791/59750 (2019).

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